INPUT ------------------------------------- FunSEQ takes BED or VCF files as input * Please prepare your input in hg19 * 1. BED format In addition to the three required BED fields, please prepare your file as follows (5 required fields, tab-delimited): chrom chromStart chromEnd Reference.allele Alterative.allele ... * chrom - The name of the chromosome (e.g. chr3, chrY). * chromStart - The starting position of the feature in the chromosome. The first base in a chromosome is numbered 0. * chromEnd - The ending position of the feature in the chromosome. The chromEnd base is not included in the display of the feature. For example, position 100 (1-based coordinate) in chromosome 1 is defined as "chr1 99 100", corresponding to position 99 in 0-based coordinate. * Reference.allele - The reference allele of SNVs * Alternative.allele - The alternative allele of SNVs. eg. To run the program properly, please strictly follow the format. Tab delimited. chr1 213941196 213941197 G T ... chr1 213942363 213942364 A C ... chr1 213943530 213943531 T A ... 2. VCF format The header line names the 8 fixed, mandatory columns. These columns are as follows (tab-delimited): #CHROM POS ID REF ALT QUAL FILTER INFO eg. To run the program properly, please strictly follow the format. Tab delimited. ##fileformat=VCFv4.0 #CHROM POS ID REF ALT QUAL FILTER INFO ... 2 4370 rs6057 G A 29 . ... ...